Title	IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia
ID	5201580
External ID	7398732491
Author	Claudia Pommerenke
Published Date	Dec. 17, 2024, 1:07 p.m.
Summary	Taken together, MKX is aberrantly expressed in subsets of AML and MM patients and cell lines thereby highlighting its likely oncogenic role in these malignancies. Due to prominent MKX expression in OC
Tags	None
URL	https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0315196
Sentiment	Negative: 0.01912 Neutral: 0.87247 Positive: 0.10841
Relevance	0.00000

Content ID	3133589
Key	660acfa9-d1a7-4562-997b-ca1090995a7e
Content	Taken together, MKX is aberrantly expressed in subsets of AML and MM patients and cell lines thereby highlighting its likely oncogenic role in these malignancies. Due to prominent MKX expression in OCI-AML3, we used this cell line as a model to investigate deregulation and function of this TALE-class homeobox gene in AML. RQ-PCR analysis confirmed enhanced MKX RNA expression in OCI-AML3, lower transcript levels in MM cell lines, and absent gene activity in AML control cell lines and primary immune and blood cells, including B-cells, T-cells, monocytes and peripheral blood mononuclear cells ( Fig 1B ). OCI-AML3 expressed even higher MKX transcript levels as compared to primary brain and prostate samples, supporting aberrant activation in this AML cell line ( Fig 1B ). Finally, Western blot analysis showed MKX expression in OCI-AML3 at the protein level, endorsing this cell line as a suitable model for MKX in AML ( Fig 1C ). Of note, screening of 32 AML cell lines using public gene expression profiling dataset GSE59808 confirmed MKX activity in OCI-AML3 while the remaining cell lines tested negative ( S3 Fig ). (A) MKX expression in 100 leukemia/lymphoma cell lines using RNA-seq data. The following entities are indicated: megakaryocytic AML, myelomonocytic AML, myeloma, and pre-B-cell ALL. MKX-positive cell lines are OCI-AML3 (AML), and KMM-1, L-363, LP-1 and OPM-2 (myeloma). (B) RQ-PCR analysis of MKX in AML and MM cell lines, and selected primary cells. The expression level of OCI-AML3 was set as 1 while the remaining samples are related to that value. (C) Western blot analysis of MKX in AML cell lines. TUBA served as loading control. Recently, we sequenced RNA from 100 leukemia/lymphoma cell lines, covering diverse hematopoietic malignancies, derived from myeloid and lymphoid lineages [ 34 ]. Screening of these RNA-seq data demonstrated prominent MKX expression in AML cell line OCI-AML3, low levels in multiple myeloma (MM) cell lines KMM-1, L-363, LP-1 and OPM-2, while cell lines derived from other entities including BCP-ALL tested negative ( Fig 1A ). Analysis of patients with corresponding malignancies using public gene expression profiling datasets showed MKX activity in about 1% of AML, and 10% of MM patients ( S2 Fig ). Thus, we identified aberrantly expressed MKX in subsets of AML and MM cell lines and patients, albeit at different frequencies. TALE-class homeobox gene IRX1 is physiologically expressed at the MEP stage of early myelopoiesis and at the pro-B-cell stage of early B-cell differentiation, while the closely related homeobox gene MKX was found to be absent in these cells following analysis of public gene expression profiling datasets GSE42519 and GSE19599 ( S1 Fig ) [ 20 , 21 ]. Furthermore, RNA-seq data from the Human Protein Atlas discounted MKX expression in mature myeloid and lymphoid hematopoietic cells, but prominent activity in brain, prostate and ovary ( S1 Fig ). Thus, in contrast to IRX1, MKX is silent in normal hematopoietic cells including MEPs and pro-B-cells. (A) Data obtained from the UCSC genome browser indicating potential TF-binding sites (blue arrow-heads) at the MKX locus. (B) RQ-PCR analysis of HOXA10 and MKX (left), IRF8 and MKX (middle), and MEF2D and MKX in OCI-AML3 treated for siRNA-mediated knockdown. (C) RQ-PCR analysis of STAT5A and MKX (left), STAT5B and MKX (middle), and GATA2 and MKX in OCI-AML3 treated for siRNA-mediated knockdown. The expression level of OCI-AML3 siCTR was set as 1 while the remaining samples are respectively related to that value. (D) ChIP-seq data from the ENCODE project showing direct binding of NFkB at the MKX locus. (E) Heatmap, based on RNA-seq data, showing gene expression levels from selected NFkB-signalling components, demonstrating elevated activity of TNFRSF11A and TNFSF11 in OCI-AML3. (F) RQ-PCR analysis of TNFRSF11A (left) and TNFSF11 (middle) in selected AML cell lines. RQ-PCR analysis of MKX in OCI-AML3 treated with TNFSF11 and NFkB-inhibitor (right). The expression level of OCI-AML3 control was set as 1 while the remaining samples are related to that value. Statistical significance was assessed by Student´s T-Test (two-tailed) and the resultant p-values indicated by asterisks (* p<0.05, p<0.01, * p<0.001, n.s. not significant). To examine the potential role of other TFs in MKX deregulation we screened TF binding sites within its gene sequence using public UCSC genome browser data ( www.genome.cseUCSC.edu ) as indicated in Fig 3A . We focused on HOXA, IRF, MEF2, STAT5, GATA and NFkB–a panel representing basic regulators of normal and malignant myelopoiesis. We performed siRNA-mediated knockdown of HOXA10 which, though reportedly regulating IRX3 and IRX5 [ 20 ], showed no regulatory impact on MKX ( Fig 3B ). MEF2C and MEF2D regulate tendon and muscle development while IRF8 and MEF2D co-operate oncogenically in AML [ 46 – 48 ]. Furthermore, RNA-seq data from AML cell lines demonstrated highly expressed IRF8 exclusively in OCI-AML3, indicating aberrant overexpression in this cell line ( S4 Fig ). However, knockdown of neither IRF8 nor MEF2D altered the expression of MKX ( Fig 3B ), excluding these factors as MKX regulators in OCI-AML3. In contrast, siRNA-mediated knockdown of STAT5A and STAT5B enhanced MKX expression ( Fig 3C ), demonstrating that these factors are transcriptional repressors of MKX. Interestingly, RNA-seq data from AML cell lines showed reduced expression levels of both STAT5A and STAT5B in OCI-AML3 ( S4 Fig ), thereby supporting MKX activity in this cell line. Next, knockdown of myeloid differentiation factor GATA2 resulted in elevated MKX expression, also demonstrating a repressive role ( Fig 3C ). Similar to STAT5, RNA-seq data showed reduced GATA2 expression in OCI-AML3 ( S4 Fig ). Finally, we investigated the role of NFkB in MKX regulation. ChIP-seq data from the ENCODE-project demonstrated direct binding of NFkB to the indicated binding site at MKX ( Fig 3D ). RNA-seq data from 23 AML cell lines for selected TNF-signalling components revealed for OCI-AML3 elevated expression of TNFSF11 and its receptor TNFRSF11A which reportedly activate NFkB TFs ( Fig 3E ) [ 49 ]. RQ-PCR analysis confirmed their high expression levels in OCI-AML3 ( Fig 3F ). While stimulation of OCI-AML3 with additional TNFSF11 spared MKX expression in OCI-AML3, treatment with a pharmacological NFkB-inhibitor suppressed MKX transcription, showing a significant activating impact of NFkB in MKX expression ( Fig 3F ). Recently, we reported aberrant expression of TALE-class homeobox genes IRX3 and IRX5 in OCI-AML3, showing that elevated BMP2-SMAD4-signalling activates IRX3 [ 20 ]. However, neither treatment with BMP2, inhibition of BMP-signalling by dorsomorphin, nor siRNA-mediated knockdown of SMAD4 altered the expression of MKX, excluding its activation via this pathway in OCI-AML3 ( Fig 2C ). In contrast, siRNA-mediated knockdown of IRX5 (but not of IRX3) resulted in slightly reduced MKX expression levels ( Fig 2D ). Furthermore, MKX expression was also reduced by knockdown of JUNB ( Fig 2E ), which has been shown to play activating roles for IRX3 and IRX5 [ 20 ]. Together, these findings showed that IRX5 and JUNB support MKX activation in OCI-AML3. Inspection of the MKX locus at the UCSC genome browser ( www.genome.cseUCSC.edu ) indicated the presence of a CpG island in the promoter region which may underlie its (de)regulated expression ( Fig 2B ). This hypothesis was supported by recent publications, reporting that DNA methylation at MKX and other homeobox genes was detected in solid tumors via a pan-cancer screen, and that OCI-AML3 carries a pediatric AML-associated mutation in DNMT3A [ 43 , 44 ]. However, treatment of AML control cell line HL-60 with DNA methyltransferase inhibitor AZA promoted activation of the accordingly regulated homeobox gene NANOG but left MKX unchanged ( Fig 2B ) [ 45 ], discounting aberrant DNA demethylation in MKX deregulation in AML. (A) Genomic copy number analysis of OCI-AML3 showing the complete chromosome 10 (above), and the chromosomal region 10p12.1-p12.3 highlighting MLLT10 and MKX (below). (B) Data obtained from the UCSC genome browser indicating a CpG-island at MKX (left). RQ-PCR analysis of MKX and NANOG in AML cell line HL-60 treated with DNA-methyltransferase inhibitor AZA (right). The expression level of HL-60 control was set as 1 while the remaining samples are related to that value. (C) RQ-PCR analysis of IRX3 and MKX in OCI-AML3 treated with BMP2 (left) and dorsomorphin (middle). RQ-PCR analysis of SMAD4, IRX3 and MKX in OCI-AML3 treated for siRNA-mediated knockdown (right). (D) RQ-PCR analysis of IRX3 and MKX (left), and IRX5 and MKX (right) in OCI-AML3 treated for siRNA-mediated knockdown. (E) RQ-PCR analysis of JUNB and MKX in OCI-AML3 treated for siRNA-mediated knockdown. The expression level of OCI-AML3 siCTR was set as 1 while the remaining samples are respectively related to that value. Statistical significance was assessed by Student´s T-Test (two-tailed) and the resultant p-values indicated by asterisks (* p<0.05, p<0.01, * p<0.001, n.s. not significant). Chromosomal rearrangements and/or genomic aberrations frequently underlie gene deregulation in hematopoietic malignancies including AML [ 39 , 40 ]. However, the reported karyotype from OCI-AML3 discounted conspicuous chromosomal abnormalities at the MKX gene located 10p12.1 ( www.DSMZ.de ). Furthermore, we performed genomic profiling of OCI-AML3, similarly discounting copy number alterations at chromosome 10, including the cryptic del(10)(p12.1-p12.3) which can generate fusion gene MLLT10::MKX, as described in pediatric BCP-ALL ( Fig 2A ) [ 41 ]. Of note, chromosomal aberration t(10;11)(p12;q23) is a frequent rearrangement in AML which also targets MLLT10 at position 10p12.3, but generates the fusion gene KMT2A::MLLT10 [ 42 ]. Thus, neither chromosomal nor genomic aberrations at position 10p12 were detected in OCI-AML3, such as might contribute to MKX activation. MKX target genes and function in AML To identify target genes regulated by MKX in AML we subjected OCI-AML3 to siRNA-mediated knockdown of MKX and subsequent RNA sequencing analysis. After confirming knockdown at transcript and protein levels (Fig 4A), the most significantly expressed genes differing between knockdowns and controls were listed, showing 78 activated and 28 genes repressed by MKX (S1 Table). For more detailed analysis we selected activated CCL2 (also known as MCP-1) which encodes a ligand that binds and activates its cognate receptor CCR2, plays multifaceted roles in myeloid immune cells, and acts as an oncogene in some cancer types [50, 51]. Knockdown of MKX in OCI-AML3 was confirmed by Western blot analysis, and the concomitant reduction of CCL2 protein in the supernatant by ELISA analysis (Fig 4A). RQ-PCR analysis showed enhanced CCL2 expression levels in AML cell lines OCI-AML3 and MUTZ-3, and corresponding expression was detected at the protein level by ELISA (Fig 4B). Thus, CCL2 is activated by MKX in OCI-AML3 and, accordingly, strongly expressed. However, forced expression of MKX in MKX-negative AML cell line NB-4 reduced CCL2 transcription (Fig 4C), indicating the absence of additional factors implicated in the regulation of CCL2 or cooperation with MKX. PPT PowerPoint slide PNG larger image TIFF original image Download: Fig 4. MKX activates CCL2 in OCI-AML3. (A) Western blot analysis of MKX in OCI-AML3 treated for siRNA-mediated knockdown of MKX. TUBA served as loading control (left). ELISA analysis of CCL2 protein in supernatants from OCI-AML3 treated for siRNA-mediated knockdown of MKX (right). (B) RQ-PCR analysis of CCL2 in selected AML cell lines (left). ELISA analysis of CCL2 protein in supernatants from selected AML cell lines (right). (C) RQ-PCR analysis of NB-4 treated for MKX-overexpression resulted in reduced CCL2 transcript levels. (D) RQ-PCR analysis of IRX3 and CCL2 (left), and IRX5 and CCL2 (right) in OCI-AML3 treated for siRNA-mediated knockdown. (E) Gene expression profiling data for CCL2 from hematopoietic stem and developing myeloid cells using public dataset GSE42519. (F) RQ-PCR analysis of CCL2 in OCI-AML3 treated for siRNA-mediated knockdown of CCL2 (left). Live-cell imaging analyses of OCI-AML3 treated for siRNA-mediated knockdown of CCL2, detecting apoptosis (right) and (G) proliferation (left). The data for the last point of imaging showed no significant difference for apoptosis but for proliferation (p = 0.0059). RQ-PCR analysis of CCL2 and CCND1 in OCI-AML3 treated for siRNA-mediated knockdown of CCL2 (right). The expression levels of NB-4 vector and OCI-AML3 siCTR were set as 1 while the remaining samples are respectively related to those values. Statistical significance was assessed by Student´s T-Test (two-tailed) and the resultant p-values indicated by asterisks (* p<0.05, p<0.01, * p<0.001, n.s. not significant). https://doi.org/10.1371/journal.pone.0315196.g004 Analysis of the CCL2 promoter region revealed three binding sites for MKX (at -1418 bp, -663 bp, -510 bp) indicating direct regulation (S5 Fig). This region also contained a binding site for IRX factors (at -574 bp) which slightly differ from that for MKX, suggesting transcriptional regulation of CCL2 by IRX3 and/or IRX5 as well (S5 Fig). Consistently, siRNA-mediated knockdown of IRX3 and IRX5 resulted in reduced CCL2 expression, confirming stimulation by both factors (Fig 4D). Thus, CCL2 is activated by aberrantly expressed TALE homeodomain TFs MKX, IRX3 and IRX5 in AML cell line OCI-AML3. Of note, CCL2 is not significantly expressed during early myelopoiesis, indicating stage-independent effects when ectopically activated (Fig 4E). Functional analysis of CCL2 was performed in OCI-AML3 via its knockdown which was confirmed by RQ-PCR (Fig 4F). Live-cell imaging analysis of the treated cells discounted any role in apoptosis (Fig 4F) but rather that of driving proliferation (Fig 4G). Consistently, siRNA-mediated knockdown of CCL2 resulted in downregulation of CCND1, demonstrating that CCL2 activates this proliferation driver in OCI-AML3 (Fig 4G). Additional MKX target genes identified by our knockdown/RNA-sequencing approach revealed activated SESN3, and repressed GATA2 (S1 Table). SESN3 (SESTRIN 3) encodes a stress-regulator which reduces intracellular reactive oxygen species (ROS), and GATA2 a myelopoietic TF [52, 53]. Regulation of these genes by MKX was confirmed by RQ-PCR analysis after siRNA-mediated knockdown (Fig 5A). In MEPs, expression profiling data from developmental stages of myelopoiesis demonstrated reduced levels of SESN3, and elevated levels of GATA2 (Fig 5B). Therefore, deregulation of these genes at that stage may disturb survival and differentiation processes during early myelopoiesis. PPT PowerPoint slide PNG larger image TIFF original image Download: Fig 5. MKX impacts apoptosis and differentiation. (A) RQ-PCR analysis of MKX and SESN3 (above) and of MKX and GATA2 (below) in OCI-AML3 treated for siRNA-mediated knockdown of MKX. (B) Gene expression profiling data for SESN3 (above) and GATA2 (below) from hematopoietic stem and developing myeloid cells using public dataset GSE42519. (C) RQ-PCR analysis of MKX in OCI-AML3 treated for siRNA-mediated knockdown of MKX (left). Analysis of apoptosis by live-cell imaging of OCI-AML3 treated for siRNA-mediated knockdown of MKX (middle, p = 0.0052) and additionally with etoposide (right, p = 0.0148). The calculated p-values refer to the last point of imaging. (D) RQ-PCR analysis of MKX and BCL2L11 in OCI-AML3 treated for siRNA-mediated knockdown of MKX. (E) Gene expression profiling data for BCL2L11 from hematopoietic stem and developing myeloid cells using public dataset GSE42519. (F) RQ-PCR analysis of HL-60 treated for MKX-overexpression resulted in reduced transcript levels of GATA2 and BCL2L11, and elevated SESN3 levels. The expression levels of OCI-AML3 siCTR and HL-60 vector were set as 1 while the remaining samples are respectively related to those values. Statistical significance was assessed by Student´s T-Test (two-tailed) and the resultant p-values indicated by asterisks (* p<0.05, p<0.01, * p<0.001, n.s. not significant). https://doi.org/10.1371/journal.pone.0315196.g005 Identification of MKX-deregulated survival factor SESN3 prompted analysis of the impact of MKX on apoptosis via knockdown and live-cell imaging. Consistent with our other findings, the data revealed a prominent role for MKX in cell survival (Fig 5C). Moreover, simultaneous treatment with apoptosis-inductor etoposide enhanced the observed MKX knockdown effect (Fig 5C). BCL2L11 encodes a fundamental activator of apoptosis, acts in the context of etoposide, and is regulatory connected with SESN3 [52, 54]. Accordingly, knockdown of MKX in OCI-AML3 resulted in BCL2L11 activation (Fig 5D), indicating that combined activation of SESN3 and suppression of BCL2L11 is responsible for the positive role of MKX in cell survival. Moreover, analysis of the promoter region of BCL2L11 revealed MKX binding sites at -1424 bp and -1291 bp (S5 Fig), suggesting direct transcriptional regulation by MKX. Interestingly, BCL2L11 is elevated during myelopoiesis at the MEP stage and may, therefore, physiologically predispose these progenitors to apoptosis (Fig 5E). Finally, forced expression of MKX in AML cell line HL-60 suppressed GATA2 and BCL2L11, and activated SESN3 (Fig 5F), confirming the regulatory impact observed in OCI-AML3. Next, we analyzed expression profiling data from MM patients comparing MKX-high with MKX-low samples using public data set GSE19554 and the associated online tool GEOR, which identifies 250 most significantly differing genes. This exercise revealed downregulated GATA2 and SESN3 in MKX-high MM patients (S6 Fig), showing target genes similar to those identified in AML, although inhibition of SESN3 contrasts with its activation by MKX in OCI-AML3. Furthermore, we found downregulated CEBPA, CEBPD and IRF8 in MKX-high MM patients (S6 Fig). These three genes are physiologically downregulated in MEPs (Fig 6A), indicating a specific role in myeloid differentiation at that developmental stage. RQ-PCR analysis of these genes in OCI-AML3 after MKX knockdown showed that MKX activated CEBPD while IRF8 and CEBPA were not regulated by this factor (Fig 6B). Forced expression of MKX in NB-4 and HL-60 resulted in elevated CEBPD (Fig 6C), confirming MKX-mediated activation of CEBPD. Thus, analysis of MKX in MM identified CEBPD as an additional aberrantly activated target gene in AML. PPT PowerPoint slide PNG larger image TIFF original image Download: Fig 6. MKX activates CEBPD. (A) Gene expression profiling data for IRF8 (above), CEBPA (middle), and CEBPD (below) from hematopoietic stem and developing myeloid cells using public dataset GSE42519. (B) RQ-PCR analysis of MKX and IRF8 (left), MKX and CEBPA (middle), and MKX and CEBPD (right) in OCI-AML3 treated for siRNA-mediated knockdown of MKX. (C) RQ-PCR analysis of NB-4 (left) and HL-60 (right) treated for MKX-overexpression resulted in elevated CEBPD transcript levels. The expression levels of OCI-AML3 siCTR, NB-4 vector and HL-60 vector were set as 1 while the remaining samples are respectively related to those values. Statistical significance was assessed by Student´s T-Test (two-tailed) and the resultant p-values indicated by asterisks (* p<0.05, p<0.01, * p<0.001, n.s. not significant). https://doi.org/10.1371/journal.pone.0315196.g006 Taken together, we identified BCL2L11, CCL2, CEBPD, GATA2 and SESN3 as MKX target genes in AML cell line OCI-AML3. These genes impact apoptosis, proliferation and differentiation, thus demonstrating several oncogenic functions for deregulated MKX in AML. Except CCL2, these MKX target genes show conspicuous activity in myelopoiesis at the IRX1-positive MEP stage, highlighting this developmental progenitor as a candidate cell of origin in AML cases aberrantly expressing IRX1-related MKX.
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PREPEND_WWW	`False`
QT_API_KEY	`'********************'`
QT_APP_ID	`'13d3cbab'`
QT_PASSWORD	`'********************'`
QT_USERNAME	`'kevin+dev3@pennyloupe.com'`
ROOT_URLCONF	`'app.urls'`
SALT_KEY	`'********************'`
SCRAPER_API_KEY	`'********************'`
SECRET_KEY	`'********************'`
SECRET_KEY_FALLBACKS	`'********************'`
SECURE_CONTENT_TYPE_NOSNIFF	`True`
SECURE_CROSS_ORIGIN_OPENER_POLICY	`'same-origin'`
SECURE_HSTS_INCLUDE_SUBDOMAINS	`False`
SECURE_HSTS_PRELOAD	`False`
SECURE_HSTS_SECONDS	`0`
SECURE_PROXY_SSL_HEADER	`None`
SECURE_REDIRECT_EXEMPT	`[]`
SECURE_REFERRER_POLICY	`'same-origin'`
SECURE_SSL_HOST	`None`
SECURE_SSL_REDIRECT	`False`
SERVER_EMAIL	`'root@localhost'`
SESSION_CACHE_ALIAS	`'default'`
SESSION_COOKIE_AGE	`1209600`
SESSION_COOKIE_DOMAIN	`None`
SESSION_COOKIE_HTTPONLY	`True`
SESSION_COOKIE_NAME	`'sessionid'`
SESSION_COOKIE_PATH	`'/'`
SESSION_COOKIE_SAMESITE	`'Lax'`
SESSION_COOKIE_SECURE	`False`
SESSION_ENGINE	`'django.contrib.sessions.backends.db'`
SESSION_EXPIRE_AT_BROWSER_CLOSE	`False`
SESSION_FILE_PATH	`None`
SESSION_SAVE_EVERY_REQUEST	`False`
SESSION_SERIALIZER	`'django.contrib.sessions.serializers.JSONSerializer'`
SETTINGS_MODULE	`'app.settings'`
SHORT_DATETIME_FORMAT	`'m/d/Y P'`
SHORT_DATE_FORMAT	`'m/d/Y'`
SIGNING_BACKEND	`'django.core.signing.TimestampSigner'`
SILENCED_SYSTEM_CHECKS	`[]`
SQL_FILES_DIR	`'/mnt/disks/pennyloupe_disk/pennyloupe/app/core/sql'`
STATICFILES_DIRS	`[]`
STATICFILES_FINDERS	`['django.contrib.staticfiles.finders.FileSystemFinder', 'django.contrib.staticfiles.finders.AppDirectoriesFinder']`
STATIC_ROOT	`PosixPath('/mnt/disks/pennyloupe_disk/pennyloupe/static')`
STATIC_URL	`'/static/'`
STORAGES	`{'default': {'BACKEND': 'django.core.files.storage.FileSystemStorage'}, 'staticfiles': {'BACKEND': 'django.contrib.staticfiles.storage.StaticFilesStorage'}}`
TEMPLATES	`[{'APP_DIRS': True, 'BACKEND': 'django.template.backends.django.DjangoTemplates', 'DIRS': ['templates'], 'OPTIONS': {'context_processors': ['django.template.context_processors.debug', 'django.template.context_processors.request', 'django.contrib.auth.context_processors.auth', 'django.contrib.messages.context_processors.messages']}}]`
TEST_NON_SERIALIZED_APPS	`[]`
TEST_RUNNER	`'django.test.runner.DiscoverRunner'`
THOUSAND_SEPARATOR	`','`
TIME_FORMAT	`'P'`
TIME_INPUT_FORMATS	`['%H:%M:%S', '%H:%M:%S.%f', '%H:%M']`
TIME_ZONE	`'UTC'`
USE_I18N	`True`
USE_THOUSAND_SEPARATOR	`False`
USE_TZ	`True`
USE_X_FORWARDED_HOST	`False`
USE_X_FORWARDED_PORT	`False`
WSGI_APPLICATION	`'app.wsgi.application'`
X_FRAME_OPTIONS	`'DENY'`
YEAR_MONTH_FORMAT	`'F Y'`

Headers

Request headers

Key	Value
Accept	/
Accept-Encoding	gzip, br, zstd, deflate
Cookie	=> see Request panel
Host	pennyloupe.com
User-Agent	Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)

Response headers

Key	Value
Content-Type	text/html; charset=utf-8

WSGI environ

Since the WSGI environ inherits the environment of the server, only a significant subset is shown below.

Key	Value
CONTENT_LENGTH
CONTENT_TYPE
PATH_INFO	/ticker/RNA/content/5201580
QUERY_STRING
REMOTE_ADDR	216.73.216.158
REQUEST_METHOD	GET
SCRIPT_NAME
SERVER_NAME	pennyloupe.com
SERVER_PORT	443
SERVER_PROTOCOL	HTTP/1.1

Request

View information

View function	Arguments	Keyword arguments	URL name
`app.core.views.app.ticker_content_detail`	`()`	`{'content_id': 5201580, 'ticker': 'RNA'}`	`ticker_content_detail`

Cookies

Variable	Value
`'csrftoken'`	`'ywO7hCQ0wcZB42TEAdwuy77kIpBPQRsm'`

No session data

No GET data

No POST data

SQL queries from 1 connection

default 11.78 ms (4 queries )

Query Timeline Time (ms) Action

SELECT "pl_content"."created_dt",
       "pl_content"."updated_dt",
       "pl_content"."status_id",
       "pl_content"."id",
       "pl_content"."key",
       "pl_content"."fetch_ref_id",
       "pl_content"."ticker",
       "pl_content"."publish_dt",
       "pl_content"."external_id",
       "pl_content"."published_by",
       "pl_content"."title",
       "pl_content"."summary",
       "pl_content"."tags",
       "pl_content"."url",
       "pl_content"."author",
       "pl_content"."author_reputation_score",
       "pl_content"."content_length",
       "pl_content"."parent_key",
       "pl_content"."sentiment_positive",
       "pl_content"."sentiment_negative",
       "pl_content"."sentiment_neutral",
       "pl_content"."sentiment",
       "pl_content"."sentiment_status",
       "pl_content"."relevance_score",
       "pl_content"."votes_positive",
       "pl_content"."votes_negative",
       "pl_content"."is_processing_flag",
       "pl_content"."vector_embedding_dt",
       "pl_content"."vector_id",
       "pl_content"."vector_status",
       "pl_content"."data_source_id",
       "pl_content"."parent_content_id",
       "pl_content"."publisher_id"
  FROM "pl_content"
WHERE "pl_content"."id" = 5201580
LIMIT 21SELECT ••• FROM "pl_content" WHERE "pl_content"."id" = 5201580 LIMIT 21

3.32

Connection: default

Transaction status: Idle

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/whitenoise/middleware.py in __call__(124)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django_htmx/middleware.py in __call__(43)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/app/core/views/app.py in ticker_content_detail(2297)
  content = Content.objects.get(id=content_id)

SELECT "pl_content_content"."created_dt",
       "pl_content_content"."updated_dt",
       "pl_content_content"."status_id",
       "pl_content_content"."id",
       "pl_content_content"."key",
       "pl_content_content"."content",
       "pl_content_content"."content_ref_url",
       "pl_content_content"."content_id"
  FROM "pl_content_content"
WHERE "pl_content_content"."content_id" = 5201580
ORDER BY "pl_content_content"."id" ASC
LIMIT 1SELECT ••• FROM "pl_content_content" WHERE "pl_content_content"."content_id" = 5201580 ORDER BY "pl_content_content"."id" ASC LIMIT 1

5.97

Connection: default

Transaction status: Idle

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/whitenoise/middleware.py in __call__(124)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django_htmx/middleware.py in __call__(43)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/app/core/views/app.py in ticker_content_detail(2303)
  content_content = ContentContent.objects.filter(details=content).first()

SELECT "pl_data_source"."created_dt",
       "pl_data_source"."updated_dt",
       "pl_data_source"."status_id",
       "pl_data_source"."id",
       "pl_data_source"."name",
       "pl_data_source"."type",
       "pl_data_source"."url",
       "pl_data_source"."description",
       "pl_data_source"."weight",
       "pl_data_source"."class_name",
       "pl_data_source"."class_params_json"
  FROM "pl_data_source"
WHERE "pl_data_source"."id" = 5
LIMIT 21SELECT ••• FROM "pl_data_source" WHERE "pl_data_source"."id" = 5 LIMIT 21

1.20

Connection: default

Transaction status: Idle

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/whitenoise/middleware.py in __call__(124)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django_htmx/middleware.py in __call__(43)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/app/core/views/app.py in ticker_content_detail(2307)
  data_source = content.data_source

SELECT COUNT(*) AS "__count"
FROM "pl_content"
WHERE "pl_content"."parent_content_id" = 5201580SELECT COUNT(*) AS "__count" FROM "pl_content" WHERE "pl_content"."parent_content_id" = 5201580

1.29

Connection: default

Transaction status: Idle

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/whitenoise/middleware.py in __call__(124)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django_htmx/middleware.py in __call__(43)
  return self.get_response(request)

/mnt/disks/pennyloupe_disk/pennyloupe/app/core/views/app.py in ticker_content_detail(2319)
  return render(request, "ticker_content_detail.html", context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/shortcuts.py in render(25)
  content = loader.render_to_string(template_name, context, request, using=using)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/loader.py in render_to_string(62)
  return template.render(context, request)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/backends/django.py in render(107)
  return self.template.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render(171)
  return self._render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/test/utils.py in instrumented_test_render(114)
  return self.nodelist.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in <listcomp>(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render_annotated(977)
  return self.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/loader_tags.py in render(159)
  return compiled_parent._render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/test/utils.py in instrumented_test_render(114)
  return self.nodelist.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in <listcomp>(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render_annotated(977)
  return self.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/loader_tags.py in render(65)
  result = block.nodelist.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in <listcomp>(1016)
  return SafeString("".join([node.render_annotated(context) for node in self]))

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in render_annotated(977)
  return self.render(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/defaulttags.py in render(320)
  match = condition.eval(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/smartif.py in eval(61)
  return func(context, self.first, self.second)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/smartif.py in <lambda>(107)
  ">": infix(10, lambda context, x, y: x.eval(context) > y.eval(context)),

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/defaulttags.py in eval(886)
  return self.value.resolve(context, ignore_failures=True)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in resolve(722)
  obj = self.var.resolve(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in resolve(854)
  value = self._resolve_lookup(context)

/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/django/template/base.py in _resolve_lookup(925)
  current = current()

27	`<td><a href="{% url 'ticker_content_detail' ticker=ticker content_id=content_detail.content.parent.id %}">{{ content_detail.content.parent.title }}</a></td>`
28	`</tr>`
29	`{% endif %}`
30	`{% if content_detail.content.children.count > 0 %}`
31	`<tr>`
32	`<th>Children</th>`
33	`<td>`
34	`<ul>`

/mnt/disks/pennyloupe_disk/pennyloupe/app/core/templates/ticker_content_detail.html

Static files (720 found, 2 used)

Static file paths

None

Static file apps

grappelli
django.contrib.admin
django_htmx
app.core
django_extensions
debug_toolbar

Static files

css/pennyloupe.css: /mnt/disks/pennyloupe_disk/pennyloupe/app/core/static/css/pennyloupe.css
img/penny_loupe_logo.png: /mnt/disks/pennyloupe_disk/pennyloupe/app/core/static/img/penny_loupe_logo.png

django.contrib.staticfiles.finders.AppDirectoriesFinder (720 files)

Templates (6 rendered)

Template path

templates

Templates

ticker_content_detail.html: /mnt/disks/pennyloupe_disk/pennyloupe/app/core/templates/ticker_content_detail.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'}
base.html: /mnt/disks/pennyloupe_disk/pennyloupe/templates/base.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'}
components/leftnav.html: /mnt/disks/pennyloupe_disk/pennyloupe/templates/components/leftnav.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'} {'block': <Block Node: leftnav. Contents: [<TextNode: '\n <div'>, <IncludeNode: template=<FilterExpression '"components/leftnav.html"'>>, <TextNode: '\n </di'>]>}
components/header.html: /mnt/disks/pennyloupe_disk/pennyloupe/templates/components/header.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'} {'block': <Block Node: header. Contents: [<TextNode: '\n\n '>, <IncludeNode: template=<FilterExpression '"components/header.html"'>>, <TextNode: '\n\n '>]>}
components/ticker_subnav.html: /mnt/disks/pennyloupe_disk/pennyloupe/app/core/templates/components/ticker_subnav.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'} {'block': <Block Node: content. Contents: [<TextNode: '\n\n <div class="uk-flex'>, <IncludeNode: template=<FilterExpression '"components/ticker_subnav.html"'>>, <TextNode: '\n <!-- /SUB NAV--'>, <Variable Node: content_detail.content.title>, <TextNode: '</td>\n '>, <IfNode>, <TextNode: '\n '>, <IfNode>, <TextNode: '\n '>, <Variable Node: content_detail.content.id>, <TextNode: '</td>\n '>, <Variable Node: content_detail.content.external_id>, <TextNode: '</td>\n '>, <Variable Node: content_detail.content.author>, <TextNode: '</td>\n '>, <Variable Node: content_detail.content.publish_dt>, <TextNode: '</td>\n '>, <Variable Node: content_detail.content.summary>, <TextNode: '</td>\n '>, <Variable Node: content_detail.content.tags>, <TextNode: '</td>\n '>, <IfNode>, <TextNode: '\n '>, <Variable Node: content_detail.content.sentiment_negative>, <TextNode: '   \n '>, <Variable Node: content_detail.content.sentiment_neutral>, <TextNode: '   \n '>, <Variable Node: content_detail.content.sentiment_positive>, <TextNode: '<br/>\n '>, <django.template.defaulttags.WidthRatioNode object at 0x7f3fb6007ed0>, <TextNode: '%;"></div>\n '>, <Variable Node: content_detail.content.sentiment_neutral>, <TextNode: '" style="width:'>, <django.template.defaulttags.WidthRatioNode object at 0x7f3fb600c310>, <TextNode: '%;"></div>\n '>, <Variable Node: content_detail.content.sentiment_positive>, <TextNode: '" style="width:'>, <django.template.defaulttags.WidthRatioNode object at 0x7f3fb600c690>, <TextNode: '%;"></div>\n '>, <Variable Node: content_detail.content.relevance_score>, <TextNode: '</td>\n '>, <IfNode>, <TextNode: '\n '>, <IfNode>, <TextNode: '\n <li clas'>, <Variable Node: content_detail.data_source.name>, <TextNode: '\n '>, <Variable Node: content_detail.data_source.id>, <TextNode: ')\n '>, <Variable Node: content_detail.data_source.type>, <TextNode: '</td>\n '>, <Variable Node: content_detail.data_source.url>, <TextNode: '" target="_blank">'>, <Variable Node: content_detail.data_source.url>, <TextNode: '</a></td>\n '>, <Variable Node: content_detail.data_source.description>, <TextNode: '</td>\n '>, <Variable Node: content_detail.data_source.weight>, <TextNode: '</td>\n '>]>} {'ticker': 'RNA'}
components/footer.html: /mnt/disks/pennyloupe_disk/pennyloupe/templates/components/footer.html; Toggle context
{'False': False, 'None': None, 'True': True} {'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'ERROR': 40, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30}, 'csrf_token': '<SimpleLazyObject: <function csrf.<locals>._get_val at ' '0x7f3fb52ab2e0>>', 'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>), 'request': '<<request>>', 'user': '<SimpleLazyObject: <function ' 'AuthenticationMiddleware.process_request.<locals>.<lambda> at ' '0x7f3fb459f380>>'} {'content_detail': {'content': <Content: IRX-related homeobox gene <i>MKX</i> is a novel oncogene in acute myeloid leukemia - Claudia Pommerenke>, 'content_content': <ContentContent: Content ID: 3133589>, 'data_source': <DataSource: Quantexa News>, 'publisher': None}, 'ticker': 'RNA'} {'block': <Block Node: footer. Contents: [<TextNode: '\n '>, <IncludeNode: template=<FilterExpression '"components/footer.html"'>>, <TextNode: '\n '>]>}

Context processors

django.template.context_processors.csrf: Toggle context
{'csrf_token': <SimpleLazyObject: <function csrf.<locals>._get_val at 0x7f3fb52ab2e0>>}
django.template.context_processors.debug: Toggle context
{}
django.template.context_processors.request: Toggle context
{'request': <WSGIRequest: GET '/ticker/RNA/content/5201580'>}
django.contrib.auth.context_processors.auth: Toggle context
{'user': <SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>, 'perms': PermWrapper(<SimpleLazyObject: <function AuthenticationMiddleware.process_request.<locals>.<lambda> at 0x7f3fb459f380>>)}
django.contrib.messages.context_processors.messages: Toggle context
{'messages': <FallbackStorage: request=<WSGIRequest: GET '/ticker/RNA/content/5201580'>>, 'DEFAULT_MESSAGE_LEVELS': {'DEBUG': 10, 'INFO': 20, 'SUCCESS': 25, 'WARNING': 30, 'ERROR': 40}}

Cache calls from 1 backend

Summary

Total calls	Total time	Cache hits	Cache misses
0	0 ms	0	0

Commands

add	get	set	get_or_set	touch	delete	clear	get_many	set_many	delete_many	has_key	incr	decr	incr_version	decr_version
0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Signals

Signal	Receivers
class_prepared
connection_created	register_type_handlers
got_request_exception
m2m_changed
post_delete
post_init
post_migrate	create_permissions, create_contenttypes
post_save	create_initial_trade_position
pre_delete
pre_init
pre_migrate	inject_rename_contenttypes_operations
pre_save
request_finished	close_old_connections, close_caches, reset_urlconf
request_started	reset_queries, close_old_connections
setting_changed	reset_cache, clear_cache_handlers, update_installed_apps, update_connections_time_zone, clear_routers_cache, reset_template_engines, storages_changed, clear_serializers_cache, language_changed, localize_settings_changed, complex_setting_changed, root_urlconf_changed, static_storage_changed, static_finders_changed, form_renderer_changed, auth_password_validators_changed, user_model_swapped, update_toolbar_config, reset_hashers, Options.setting_changed, update_level_tags, uninstall_if_needed, clear_caches, StaticFilesStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties, FileSystemStorage._clear_cached_properties

Path	Location
.DS_Store	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/.DS_Store
grappelli/.DS_Store	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/.DS_Store
grappelli/js/grappelli.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/grappelli.js
grappelli/js/jquery.grp_related_m2m.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_related_m2m.js
grappelli/js/jquery.grp_autocomplete_fk.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_autocomplete_fk.js
grappelli/js/jquery.grp_autocomplete_generic.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_autocomplete_generic.js
grappelli/js/jquery.grp_timepicker.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_timepicker.js
grappelli/js/jquery.grp_collapsible_group.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_collapsible_group.js
grappelli/js/jquery.grp_related_generic.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_related_generic.js
grappelli/js/jquery.grp_related_fk.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_related_fk.js
grappelli/js/jquery.grp_collapsible.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_collapsible.js
grappelli/js/grappelli.min.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/grappelli.min.js
grappelli/js/jquery.grp_autocomplete_m2m.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_autocomplete_m2m.js
grappelli/js/jquery.grp_inline.js	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/js/jquery.grp_inline.js
grappelli/images/spritesheet-1694777276.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/spritesheet-1694777276.png
grappelli/images/backgrounds/loading-small.gif	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/backgrounds/loading-small.gif
grappelli/images/backgrounds/changelist-results.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/backgrounds/changelist-results.png
grappelli/images/backgrounds/ui-sortable-placeholder.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/backgrounds/ui-sortable-placeholder.png
grappelli/images/backgrounds/nav-grabber.gif	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/backgrounds/nav-grabber.gif
grappelli/images/backgrounds/messagelist.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/backgrounds/messagelist.png
grappelli/images/icons/ui-datepicker-next.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/ui-datepicker-next.png
grappelli/images/icons/link-external-small_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/link-external-small_hover.png
grappelli/images/icons/tools-arrow-up-handler_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-arrow-up-handler_hover.png
grappelli/images/icons/date-hierarchy-back.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/date-hierarchy-back.png
grappelli/images/icons/tools-delete-handler-predelete.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-delete-handler-predelete.png
grappelli/images/icons/breadcrumbs-rtl.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/breadcrumbs-rtl.png
grappelli/images/icons/datepicker_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/datepicker_hover.png
grappelli/images/icons/tools-trash-handler_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-trash-handler_hover.png
grappelli/images/icons/selector-add-m2m-vertical_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/selector-add-m2m-vertical_hover.png
grappelli/images/icons/tools-related-remove-handler-small_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-related-remove-handler-small_hover.png
grappelli/images/icons/sorted-descending.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/sorted-descending.png
grappelli/images/icons/breadcrumbs_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/breadcrumbs_hover.png
grappelli/images/icons/tools-viewsite-link_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-viewsite-link_hover.png
grappelli/images/icons/object-tools-add-link.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/object-tools-add-link.png
grappelli/images/icons/tools-related-edit-handler-small-disabled.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-related-edit-handler-small-disabled.png
grappelli/images/icons/tools-related-remove-handler-small-disabled.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-related-remove-handler-small-disabled.png
grappelli/images/icons/sorted-ascending.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/sorted-ascending.png
grappelli/images/icons/link-internal-rtl-small_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/link-internal-rtl-small_hover.png
grappelli/images/icons/pulldown-handler_selected.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/pulldown-handler_selected.png
grappelli/images/icons/tools-delete-handler_hover.png	/mnt/disks/pennyloupe_disk/pennyloupe/.venv/lib/python3.11/site-packages/grappelli/static/grappelli/images/icons/tools-delete-handler_hover.png

Content Detail

Versions

Time

Resource usage

Browser timing

Settings from app.settings

Headers

Request headers

Response headers

WSGI environ

Request

View information

Cookies

No session data

No GET data

No POST data

SQL queries from 1 connection

Static files (720 found, 2 used)

Static file paths

Static file apps

Static files

django.contrib.staticfiles.finders.AppDirectoriesFinder (720 files)

Templates (6 rendered)

Template path

Templates

Context processors

Cache calls from 1 backend

Summary

Commands

Signals